1. J. Diao. Epigenetic quantification using DNA hybridization-based single-molecule immunofluorescent imaging. International patent publication WO2022192510A1 (2022).

  2. J. Diao. Super-resolution morphology-correlated detection of labile zinc. International patent publication WO2021243176A1 (2021).

  3. J. Diao, C.-X. Song. Single-molecule epigenetic localization. International patent publication WO2020081629A1 (2020).

  4. C.-X. Song, S.R. Quake, A.T. Brunger, J. Diao. Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. U.S. patent publication US20170298422A1 & US20220403449A1 (2022).

  5. M.E. Reeves, J. Diao. Method of making nanoparticle wires. U.S. patent 7829139 (2010).


  • K. Qiu, W. Zou, Z. Tian, T. Huang, N-.P. Tsai, K. Zhang, J. Diao. Enhancing mitochondrial functions by optogenetic clustering. bioRxiv 2022.11.22.517578

  • B. Bu, Z. Tian, D. Li, K. Zhang, B. Ji, J. Diao. Double-transmembrane domain reduces fusion by increasing lipid-protein mismatch. bioRxiv 2020.08.24.264663

  • X. Hu, W. Hao, B. Qin, Z. Tian, Z. Li, P. Hou, R. Zhao, S. Cui, J. Diao. Mechanism of duplex unwinding by coronavirus nsp13 helicases. bioRxiv 2020.08.02.233510

Recent representative publications (as a senior author)

H. Fang, L. Hu, Q. Chen, S. Geng, C. Wang, M. Hao, Z. Tian, H. Chen, L. Liu, J.-L. Guan, Y. Chen, L. Dong, Z. Guo, W. He, J. Diao. An ER-targeted “reserve-release” fluorogen for topological quantification of reticulophagy. Biomaterials 292, 121929 (2023).

K. Qiu, W. Zou, H. Fang, M. Hao, K. Mehta, Z. Tian, J.-L. Guan, K. Zhang, T. Huang, J. Diao. Light-activated mitochondrial fission through optogenetic control of mitochondria-lysosome contacts. Nat. Commun. 13, 4303 (2022).

K. Qiu, R. Seino, G. Han, M. Ishiyama, Y. Ueno, Z. Tian, Y. Sun, J. Diao. De novo design of a membrane-anchored probe for multi-dimensional quantification of endocytic dynamics. Adv. Healthc. Mater. 11, 2102185 (2022).

Q. Chen, M. Hao, L. Wang, L. Li, Y. Chen, X. Shao, Z. Tian, R.A. Pfuetzner, Q. Zhong, A.T. Brunger, J.-L. Guan, J. Diao. Prefused lysosomes cluster on autophagosomes regulated by VAMP8. Cell Death Dis. 12, 939 (2021).

K.-N. Wang, X. Shao, Z. Tian, L.-Y. Liu, C. Zhang, C.-P. Tan, J. Zhang, P. Ling, F. Liu, Q. Chen, J. Diao, Z.-W. Mao. A continuously add-on probe reveals a nonlinear enlargement of mitochondria in light-activated oncosis. Adv. Sci. 8, 2004566 (2021).

Y. Du, Y. Lai, J. Liu, J. Diao. Epigenetic quantification of DNA 5-hydroxymethylcytosine using DNA hybridization-based single-molecule immunofluorescent imaging. Small Methods 5, 2100061 (2021).

H. Fang, S. Geng, M. Hao, Q. Chen, M. Liu, C. Liu, Z. Tian, C. Wang, T. Takebe, J.-L. Guan, Y. Chen, Z. Guo, W. He, J. Diao. Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells. Nat. Commun. 12, 109 (2021).

Q. Chen, H. Fang, X. Shao, Z. Tian, S. Geng, Y. Zhang, H. Fan, P. Xiang, J. Zhang, X. Tian, K. Zhang, W. He, Z. Guo, J. Diao. A dual-labeling molecular probe to track functional mitochondria-lysosome interactions in live cells. Nat. Commun. 11, 6290 (2020).

L.-Y. Liu, H. Fang, Q. Chen, M. H.-Y. Chan, M. Ng, K.-N. Wang, W. Liu, Z. Tian, J. Diao, Z.-W. Mao, V. W.-W. Yam. Multiple-color platinum complex with super-large Stokes shift for super-resolution imaging of autolysosome escape. Angew. Chem. Int. Ed. 59, 19229 (2020).

Q. Chen, X. Shao, M. Hao, R. Guan, Z. Tian, M. Li, C. Wang, L. Ji, H. Chao, J.-L. Guan, J. Diao. Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy. Biomaterials 250, 120059 (2020).

H. Fang, S. Yao, Q. Chen, C. Liu, Y. Cai, S. Geng, Y. Bai, Z. Tian, A.L. Zacharias, T. Takebe, Y. Chen, Z. Guo, W. He, J. Diao. De novo-designed near-infrared nanoaggregates for super-resolution monitoring of lysosomes in cells, in whole organoids, and in vivo. ACS Nano 13, 14426 (2019).

J. Khamo, V. Krishnamurthy, Q. Chen, J. Diao, K. Zhang. Optogenetic delineation of receptor tyrosine kinase subcircuits during PC12 cell differentiation. Cell Chem. Biol. 26, 400 (2019).

Q. Chen, C. Jin, X. Shao, R. Guan, Z. Tian, C. Wang, F. Liu, P. Ling, J. Guan, L. Ji, F. Wang, H. Chao, J. Diao. Super-resolution tracking of mitochondrial dynamics with an Iridium(III) luminophore. Small 14, 1802166 (2018).

A. Bhattacharjee, Y. Wang, J. Diao, C.M. Price. Dynamic DNA binding, junction recognition and G4 melting activity underlie the telomeric and genome-wide roles of human CST. Nucleic Acids Res. 45, 12311 (2017).

J. Gong, Y. Lai, X. Li, M. Wang, J. Leitz, Y. Hu, Y. Zhang, U.B. Choi, D. Cipriano, R.A. Pfuetzner, T.C. Südhof, X. Yang, A.T. Brunger, J. Diao. C-terminal domain of mammalian complexin-1 localizes to highly curved membranes. Proc. Natl. Acad. Sci. USA 113, E7590 (2016).

Y. Lai, U.B. Choi, Y. Zhang, R.A. Pfuetzner, A.L. Wang, J. Diao, A.T. Brunger. N-terminal domain of complexin independently activates calcium-triggered fusion. Proc. Natl. Acad. Sci. USA 113, E4698 (2016).

C.-X. Song, J. Diao, A.T. Brunger, S.R. Quake. Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. Proc. Natl. Acad. Sci. USA 113, 4338 (2016).